Journal: Lab on a Chip

Article Title: A liver microphysiological system of tumor cell dormancy and inflammatory responsiveness is affected by scaffold properties

doi: 10.1039/C6LC01171C

Figure Lengend Snippet: Fig. 2 Hydrogel scaffolds created a healthier physiological microenvironment and enhanced the responsiveness of hepatic tissue. (A and B) Levels of secreted analytes (pg mL−1) in day 15 effluent from hepatic tissue supported by either polystyrene or hydrogel scaffolds were measured; 53 analytes were above the LoD (n = 5 donors). (A) Scatter plot depicts levels of secreted analytes in hepatic tissue supported by polystyrene vs. hydrogel scaffolds. Significantly altered analytes (black circles) via non-parametric pairwise Wilcoxon rank sum tests (p < 0.05): IL-12p70, IL-13, IL-15, SDF-1α/β and VEGF-A, and those with a >1.5 ratio change (red circles). (B) Graphs summarize analytes exhibiting marked ratio changes >1.5 in polystyrene (top) or hydrogel (bottom) scaffolds. (C) Immunofluorescent images of hepatic tissue formed in polystyrene or hydrogel scaffolds on day 15, obtained using a 20× objective. Red – α-SMA; blue – DAPI. (D and E) Fold changes in acute phase protein production (A1AT and fibrinogen) fol- lowing stimulation with LPS. Protein levels were measured by ELISA on day 15 following stimulation with 1 μg mL−1 LPS for 24 hours (n = 2 do- nors each in duplicate). Fold change was determined by for each pa- tient donor by normalizing each stimulated group to its respective unstimulated control group. Significant differences were determined via non-parametric pairwise Wilcoxon rank sum tests (*p < 0.05).

Article Snippet: Alpha-1-antitrypsin (A1AT) and fibrinogen secretion from hepatic tissue was measured using Enzyme-linked immunosorbent assays (ELISAs) kits according to the manufacturer's instructions (Genway Biotech Inc, San Diego, CA).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: bioRxiv

Article Title: C-type lectin-like receptor 2 (CLEC-2)-dependent DC migration is controlled by tetraspanin CD37

doi: 10.1101/227918

Figure Lengend Snippet: (A) WT (left), Cd37-/- (middle) or CD11c ΔCLEC”2 (right) DCs were stimulated in a 3D collagen gel with (bottom row) or without (upper row) recombinant podoplanin-Fc (rPDPN-Fc). Cells were stained for F-actin (red) and nucleus (blue) and imaged with a Leica SP5 confocal fluorescence microscope. One representative cell is shown for each condition (overview with more cells is provided in Supplementary Figure 2). Scale bar = 10μm. (B-D) Number (B) and length (C) of actin protrusions, and morphology index (D) of WT (left), Cd37-/- (middle) or CD11c ΔCLEC ” 2 (right) BMDCs upon rPDPN-Fc stimulation (grey boxes) compared to no ligand (white boxes). Data are shown as Tukey Box & whiskers from 3 independent experiments, total n=3 mice per genotype. In Tukey Box & whiskers, black dots are determined as outliers; i.e. data points outside the 25 th and 75 th percentile, minus or plus the 1.5 interquartile range, respectively. Two-way ANOVA with Tukey’s multiple comparisons, ***p<0.001, ****p<0.0001.

Article Snippet: BMDCs (0.3x10 6 ) were seeded into a 3D collagen (type I, rat tail)/matrigel matrix (both from Corning, via Thermo Fisher Scientific, Loughborough, UK) supplemented with 10% minimum essential medium alpha medium (MEMalpha, Invitrogen, via Thermo Fisher Scientific, Loughborough, UK) and 10% FCS (Greiner Bio-One, Stonehouse, UK) on glass-bottomed cell culture plates (MatTek Corporation, Bratislava, Slovakia).

Techniques: Recombinant, Staining, Fluorescence, Microscopy